Journal: Human Mutation

Article Title: Cross‐Cohort Transcriptomic Integration Identifies IFIT2 as a Translational Diagnostic Biomarker and Functional Driver of Inflammation‐Linked Tubular Injury in Chronic Kidney Disease

doi: 10.1155/humu/8282277

Figure Lengend Snippet: Induction and knockdown of IFIT2 in renal tubular epithelial cells. (A–B) IFN‐ γ –induced IFIT2 expression in HK‐2 and RPTEC cells. (C–D) TGF‐ β 1–induced IFIT2 expression in HK‐2 and RPTEC cells. (E–F) Validation of IFIT2 knockdown efficiency by qPCR. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Human renal proximal tubular epithelial cells, including the HK‐2 cell line (ATCC, RRID: CVCL_0302) and primary RPTEC cells (ATCC, RRID: CVCL_K278), were used in this study.

Techniques: Knockdown, Expressing, Biomarker Discovery

Journal: Human Mutation

Article Title: Cross‐Cohort Transcriptomic Integration Identifies IFIT2 as a Translational Diagnostic Biomarker and Functional Driver of Inflammation‐Linked Tubular Injury in Chronic Kidney Disease

doi: 10.1155/humu/8282277

Figure Lengend Snippet: IFIT2 knockdown attenuates IFN‐ γ –induced injury and apoptosis in renal tubular epithelial cells. (A–B) CCK‐8 assay showing that IFIT2 knockdown alleviates IFN‐ γ –induced reduction of cell viability in HK‐2 and RPTEC cells. (C–F) Annexin V/PI flow cytometry analysis showing that IFIT2 knockdown reduces IFN‐ γ –induced apoptosis in (C, E) HK‐2 and (D, F) RPTEC cells. Data are presented as mean ± SD from three independent experiments. ∗∗∗ p < 0.001.

Article Snippet: Human renal proximal tubular epithelial cells, including the HK‐2 cell line (ATCC, RRID: CVCL_0302) and primary RPTEC cells (ATCC, RRID: CVCL_K278), were used in this study.

Techniques: Knockdown, CCK-8 Assay, Flow Cytometry

Journal: Genes, chromosomes & cancer

Article Title: Genomic and metabolic characterization of a chromophobe renal cell carcinoma cell line model (UOK276)

doi: 10.1002/gcc.22476

Figure Lengend Snippet: Metabolic analysis of the UOK276 cell line. (A) Most chromophobe RCCs demonstrate increased expression of the genes encoding the components of the electron transport chain (ETC), while clear cell RCCs show decreased expression. TaqMan® mRNA expression analysis for a select number of ETC genes representing ETC complex I (NDUFA3, NDUFA6), ETC complex III (UQCRB), ETC complex IV (COX5A, COX6C), and ETC complex V (ATP5B) was performed to compare the UOK276 cell line with cell lines representing both normal human kidney (HRCE and RPTEC) and clear cell RCC (UOK139). Contrary to expectation, the UOK276 cell line demonstrated lower expression levels of the ETC genes than the normal human kidney cell lines and had an expression pattern that was more similar to the clear cell RCC cell line. (B) The oxygen consumption rate (OCR) for UOK276 was evaluated using a Seahorse XF96 Extracellular Flux Analyzer and compared with three normal kidney control cell lines (HK-2, PSC-400 and HRCE). The OCR was measured for 90 min and a comparison graph at the 60-min time point demonstrates a lower rate of oxygen consumption in the UOK276 cells that is consistent with the decreased expression of the ETC complex genes.

Article Snippet: The immortalized normal kidney cell line HK-2 and the normal human primary renal proximal tubule epithelial cells PSC-400 were purchased from the American Type Culture Collection (ATCC) and the normal human renal cortical epithelial cells HRCE were purchased from Lonza (Lonza Inc., NJ).

Techniques: Expressing, Control, Comparison